SOC Outgrowth Medium from New England Biolabs

Product Specs
ItemSOC Outgrowth Medium
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberB9020S
Quantity100 ml
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
Temperature4 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Targeted mutagenesis and high-throughput screening of diversified gene and promoter libraries for isolating gain-of-function mutationsFrontiers in Bioengineering and BiotechnologyJuly 17, 2023Herbert M. Huttanus, Ellin-Kristina H. Triola, Jeanette C. Velasquez-Guzman, Sang-Min Shin, Rommel S. Granja-Travez, Anmoldeep Singh, Taraka Dale, Ramesh K. JhaAssembled plasmid DNA library, user supplied.• SOC Outgrowth Medium (New England Biolabs, cat. no. B9020S,• Bacterial growth and selection medium (liquid and agar) e.g., Luria Broth Base (Thermo Fisher Scientific,

(2) Fibertools: fast and accurate DNA-m6A calling using single-molecule long-read sequencingbioRxivJuly 12, 2023Anupama Jha, Stephanie C. Bohaczuk, Yizi Mao, Jane Ranchalis, Benjamin J. Mallory, Alan T. Min, Morgan O. Hamm, Elliott Swanson, Connor Finkbeiner, Tony Li, et al.back on ice for 5 minutes. Following incubation, 950 μL of room temperature SOC media (NEB cat. no. B9020S) was added, and the cells were allowed to outgrow for 1 hour in the absence of selection. 50 μL of the

(3) Engineering highly thermostable Cas12b via de novo structural analyses for one-pot detection of nucleic acidsCell Reports MedicineMay 16, 2023Long T. Nguyen, Santosh R. Rananaware, Lilia G. Yang, Nicolas C. Macaluso, Julio E. Ocana-Ortiz, Katelyn S. Meister, Brianna L.M. Pizzano, Luke Samuel W. Sandoval, Raymond C. Hautamaki, Zoe R. Fang, et al.Protocols,http://cshprotocols.cshlp.org/content/2015/9/pdb.rec085894.full?rss=1 SOC Medium,New England Biolabs,B9020S Antifoam 204,Millipore Sigma,Cat# A8311-50ML NEBuffer 2⋅1,New England Biolabs,Cat# B9200S 10X Nuclease-Free

(4) Effect of mTOR inhibitors on sodium taurocholate cotransporting polypeptide (NTCP) function in vitroFrontiers in PharmacologyMarch 22, 2023Chitra Saran, Henry Ho, Paavo Honkakoski, Kim L. R. Brouwerng of the Flp recombinase expression plasmid pOG44 (Invitrogen, CA) in super-optimal broth (S.O.C.; #B9020S, New England Biolabs) using the manufacturer’s protocol. Ampicillin-resistant bacterial colonies were

(5) Coordination of bacterial cell wall and outer membrane biosynthesisNatureMarch 1, 2023Hummels KR, Berry SP, Li Z, Taguchi A, Min JK, Walker S, Marks DS, Bernhardt TG.µl of NEB-5-alpha electrocompetent E. coli (NEB C2989K), and recovered in SOC outgrowth medium (NEB B9020S), and transformants were selected by plating the outgrowth onto LB agar supplemented with gentamycin.

(6) Whole-genome functional characterization of RE1 silencers using a modified massively parallel reporter assayCell GenomicsJanuary 11, 2023Kousuke Mouri, Hannah B. Dewey, Rodrigo Castro, Daniel Berenzy, Susan Kales, Ryan Tewheyefficiency. Library E was transformed into 5-alpha E.coli (NEB, C2987H), recovered in SOC medium (NEB, B9020S) and incubated at 37°C for 1 h then diluted and plated onto LB agar supplemented with 100 μg/mL of carbenicillin

(7) An Infectious Bursal Disease Virus (IBDV) Reverse Genetics Rescue System and Neutralization Assay in Chicken B CellsCurrent ProtocolsJanuary 9, 2023Vishwanatha R. A. P. Reddy, Salik Nazki, Amin Asfor, Andrew J. BroadbentEngland Biolabs: NEB® 5‐alpha competent E. coli; C2984H, high efficiency)SOC medium (New England Biolabs: B9020S)LB agar plate supplemented with 5 μg/ml ampicillinLB broth supplemented with 5 μg/ml ampicillin (Gibco,

(8) Adaptation delay causes a burst of mutations in bacteria responding to oxidative stressEMBO reportsJanuary 9, 2023Valentine Lagage, Victor Chen, Stephan UphoffkV electric pulse for 5 ms, Bio‐Rad micropulser). Cells were recovered in SOC outgrowth medium (NEB B9020S) for 1 h at 37°C before selection on kanamycin plates. pKD46 plasmid was cured by streaking single colonies

(9) Genome-wide base editor screen identifies regulators of protein abundance in yeastElifeNovember 3, 2022Olga T Schubert, Joshua S Bloom, Meru J Sadhu, Leonid Kruglyak(Bio-Rad Laboratories #1652100). After 60 min of growth in liquid recovery media (SOC outgrowth media, NEB #B9020S), cells were plated onto five 150 mm LB agar plates supplemented with ampicillin/carbenicillin (100

(10) Functional expression of diverse post-translational peptide-modifying enzymes in Escherichia coli under uniform expression and purification conditionsPLOS ONESeptember 19, 2022Emerson Glassey, Andrew M. King, Daniel A. Anderson, Zhengan Zhang, Christopher A. Voigtliquid media (S0210, Teknova, Hollister, CA, USA) was used for making competent cells. SOC liquid media (B9020S, New England BioLabs, Iwsich, MA, USA) was used for outgrowth. Cells were induced with the following

(11) Chromosomal integration of complex DNA constructs using CRAGE and CRAGE-Duet systemsSTAR ProtocolsSeptember 16, 2022Zhiying Zhao, Jan-Fang Cheng, Yasuo YoshikuniHigh-Fidelity PCR Master Mix (2×),New England Biolabs,Cat# M0513S SOC medium,New England Biolabs,Cat# B9020S Sybr Safe,Invitrogen,Cat# S33102 "TAE, 50×",Growcells,Cat# MRGF-4210 Trace mineral solution,ATCC,Cat#

(12) Polymeric nanoparticles for dual-targeted theranostic gene delivery to hepatocellular carcinomaScience AdvancesJuly 22, 2022Hannah J. Vaughan et al.of the ligation product. Bacteria were cultured in 450 μl of SOC outgrowth medium (NEB, catalog no. B9020S) for 1 hour at 37°C. The full 500 μl of the bacteria suspension was streaked in an LB agar plate with

(13) Heterologous expression and purification of encapsulins in Streptomyces coelicolorMethodsXJuly 16, 2022Michael P. Andreas, Tobias W. Giessen(Invitrogen, catalog number 18427013).(14)SOC outgrowth medium (New England Biolabs, catalog number: B9020S).(15)LB agar, Miller powder (Fisher Scientific, catalog number: BP1425-2).(16)LB broth, Miller mix (Fisher

(14) Clamping, Bending, and Twisting Inter-Domain Motions in the Misfold-Recognising Portion of UDP-Glucose: Glycoprotein Glucosyl-TransferaseSSRN Electronic JournalMarch 30, 2022Carlos P. Modenutti et al.transfection reagent ThermoFisher Scientific Cat# 16447100 SOC Media New England Biolabs Cat# B9020S Kifunensine Cayman Chemical Cat# 109944-15-2 Carbenicillin Sigma-Aldrich Cat# C1389 CtUGGT Ref.

(15) XACT-seq: A photocrosslinking-based technique for detection of the RNA polymerase active-center position relative to DNA in Escherichia coliSTAR ProtocolsDecember 17, 2021Chirangini Pukhrambam, Irina O. Vvedenskaya, Bryce E. NickelsBiotech,Cat#R-120-25 H-Bpa-OH,Bachem,Cat#F-2800 IPTG Gold,Biotech,Cat#I2481C50 SOC Outgrowth Medium,NEB,Cat#B9020S Lysozyme Egg White,Gold Biotech,Cat#L-040-10 "Glycerol, nuclease free",VWR,Cat#EM 4750 Tris base

(16) Generation and validation of CRISPR-engineered human natural killer cell lines for research and therapeutic applicationsSTAR protocolsDecember 17, 2021Kumar A, Lee SJ, Liu Q, Chan AKN, Pokharel SP, Yu J, Chen CW, Swaminathan S.Memorial Institute)-1640,Thermo Fisher Scientific,Cat# 11875119 SOS medium,New England Biolabs,Cat# B9020S Terrific Broth (granulated),Fisher Scientific,Cat# BP9728-500 PowerUp SYBR Green Master Mix,Thermo Fisher Scientific

(17) Assembly of Longâ€Adapter Singleâ€Strand Oligonucleotide (LASSO) Probes for Massively Parallel Capture of Kilobase Size DNA TargetsCurrent ProtocolsDecember 2, 2021Lamia Chkaiban, Lorenzo Tosi, Biju Parekkadan(Thermo Fischer Scientific, cat. no. 15585011) SOC outgrowth medium (New England BioLabs, cat. no. B9020S) PureLink Quick Plasmid Miniprep Kit (thermos Scientific, cat. no. K210010) Difco, LB Broth Miller

(18) Selection for constrained peptides that bind to a single target proteinNature CommunicationsDecember 1, 2021Andrew M. King et al.maintenance. SOB liquid media (S0210, Teknova, CA) was used for making competent cells. SOC liquid media (B9020S, New England BioLabs, MA) was used for outgrowth. Unless noted otherwise, cells were induced with the

(19) Tumor-derived exosomes drive immunosuppressive macrophages in a pre-metastatic niche through glycolytic dominant metabolic reprogrammingCell MetabolismOctober 5, 2021Samantha M. Morrissey et al.K1876-1G Lennox LB microbial growth medium,Sigma,Cat# L3022 SOC outgrowth medium,New England Biolabs,Cat# B9020S Critical Commercial Assay,Critical Commercial Assay,Critical Commercial Assay Exo-Check Exosome Antibody

(20) Genome-wide functional screen of 3′UTR variants uncovers causal variants for human disease and evolutionCellSeptember 30, 2021Dustin Griesemer et al.200 ohm, 25 μF). Electroporated bacteria were immediately split into five 1 mL aliquots of SOC (NEB, B9020S) and recovered for 1 hour at 37°C then independently expanded in 1L of LB supplemented with 50 μg/mL

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(1)

1/20/2023Easy Cloning with SOC Outgrowth Medium Scarlette CASSALI use SOC Outgrowth Medium to allow my bacteria to recover and give them time to express the antibiotic gene resistance after transformation by heat shock.
(1)

10/10/2019Bacterial Cell Recovery Growth Media Atul SinghI used this outgrowth recovery media after each transformed cell. Earlier, I used LB medium for recovery, but this NEB SOC provided best results in comparison to LB.